(3)長期保存性の向上をめざして！

　食品を長期にわたり保存する手段として冷凍保存(冷凍食品)は有効です。冷凍保存は動物の胚や植物組織の保存にも使われています。これら生体材料は食品に比べ冷凍に対する感受性が高く、凍結耐性を解明するための有効なモデルとなります。そこで、食品冷凍技術のより一層の改善を目指し、凍結保存耐性を解明するために植物細胞や組織の凍結保存を行っています。

これらの実現に向け、植物培養細胞や組織(スギナ、キノアなど)、また食品素材である餡などを実験材料として、生理生化学レベル・細胞分子生物学レベル・形態分析学レベルでの研究を行っています。

発表論文

1. Kuriyama, A., Watanabe, K. and Yamana, M., Effect of glycine and leucine on the recovery of frozen-thawed Lavandula vera cells. Cryo-Letters, 21, 157-162 (2000).
2. Watanabe, K., Kuriyama, A., Kawai, F. and Kanamori, M., Effect of cryoprotectant treatment and post-thaw washing on the survival of cultured rice (Oryza sativa L.) cells after cryopreservation. Cryo-Letters, 20, 377-382 (1999).
3. 栗山昭, 渡辺克美, 凍結保存したイネ培養細胞の生存活性におよぼすアミノ酸の効果., 日作紀., 68, 275-277 (1999).
4. Watanabe, K., Kuriyama, A., Kawai, F. and Kanamori, M., Survival of rice non-embryogenic callus cells after cryopreservation in liquid nitrogen by vitrification. Plant Biotech., 15, 35-37 (1998).
5. Watanabe, K., Kuriyama, A., Kawai F. and Kanamori, M., Stability of high cryoprotectability of Lavandula vera cell lines selected by repeating a freeze-thaw procedure. Cryobiology and Cryotechnology, 43, 83-88 (1997).
6. Kuriyama, A., Kawata, K., Watanabe, K., Kawai F. and Kanamori, M., Amino acids differ in their effect on the post-thaw recovery of cryopreserved Lavandula vera cells. Hort Science, 32, 327 (1997).
7. 栗山昭, 川田訓平, 河合文雄, 金森正雄, 渡辺克美, 前田桝夫, 凍結保存したイネ培養細胞からのプロトプラスト収量の変動, 日作紀., 66, 133-134 (1997)
8. Kuriyama, A., Watanabe, K., Kawata, K., Kawai F. and Kanamori, M., Sensitivity of cryopreserved Lavandula vera cells to ammonium ion. J. Plant Physiol., 148, 693-695 (1996).
9. Watanabe K. and Steponkus, P. L., Vitrification of Oryza sativa L. cell suspensions. Cryo-Letters, 16, 255-262 (1995).
10. Watanabe, K., Kawai, F. and Kanamori, M., Factors affecting cryoprotectability of cultured rice (Oryza sativa L.) cells ---Cell wall and cell-aggregate size---. Cryo-Letters, 16, 147-156 (1995).
11. Watanabe, K., Sato, F., Yamada, Y., Kawai, F., Kanamori M. and Mitsuda, H., Characterization of polypeptides in cultured rice cells differing in cryoprotectability. J. Plant Physiol., 139, 443-447 (1992).
12. Watanabe, K., Kawai, F., Kanamori, M. and Mitsuda, H., Preservation of rice callus by gas-replacement. Plant Tissue Culture Letters, 8, 36-38 (1991).
13. Kuriyama, A., Watanabe, K., Ueno, S. and Mitsuda, H., Effect of post-thaw treatment on the viability of cryopreserved Lavandula vera cells. Cryo-Letters, 11:, 171-178 (1990).
14. Watanabe, K., Ueno, S. and Mitsuda, H., Differences in viability after freeze-preservation in liquid nitrogen among rice callus lines. Agric. Biol. Chem., 54, 1295-1296 (1990).
15. Watanabe, K., Ueno, S. and Mitsuda, H., Alterations of plasma membrane phospholipids in cultured Lavandula vera cells different in cryoprotection by a cryoprotectant treatment. Plant Cell Physiol., 31, 163-166 (1990).
16. Kuriyama, A., Watanabe, K., Ueno, S. and Mitsuda, H., Inhibitory effect of ammonium ion on recovery of cryopreserved rice cells. Plant Sci., 64, 231-235 (1989).
17. Ueno, S., Watanabe, K., Yoshida, S. and Mitsuda, H., Relationship between thermotrophic properties of isolated plasma membrane vesicles and freezing resistance of cultured green Lavandula vera cells. Proc. Japan Acad., 63 Ser B, 211-214 (1987).
18. Watanabe, K., Kito, M., Ueno, S. and Mitsuda, H., Relationships of membrane phospholipids to freezing resistance of cultured green Lavandula vera cells. Agric. Biol. Chem., 50, 1791-1796 (1986).
19. Watanabe, K., Yamada, Y., Ueno, S. and Mitsuda, H., Change of freezing resistance and retention of metabolic and differentiation potentials in cultured green Lavandula vera cells which survived repeated freeze-thaw procedures. Agric. Biol. Chem., 49, 1727-1731 (1985).
20. Watanabe, K., Mitsuda, H. and Yamada, Y., Retention of metabolic and differentiation potentials of green Lavandula vera callus after freeze-preservation. Plant & Cell Physiol., 24. 119-122 (1983).