研究業績
総説および著書：
大沼貴之
：キチン質分解酵素の構造と機能および利用に関する研究、応用糖質科学, 6(1)15-20 (2016)
北奥喜仁, 大沼貴之 ：等温滴定型カロリメトリーを用いた生体分子間相互作用解析、化学と生物, 53(12)834-842 (2015)
大沼貴之 ：椊物キチナーゼの構造と機能、その利用、応用糖質科学, 4(2)121 (2014)
深溝 慶 ：キチン質の酵素分解とオリゴ糖合成研究の新展開―緒言―キチン質多糖の分解とオリゴ糖合成、応用糖質科学, 4(2)103-105 (2014)
新家粧子、深溝 慶 ：CBM32ファミリーに属する糖質結合モジュールの構造と特異性、キチン・キトサン研究Chitin and Chitosan Research, 2014, 20(1), 38-43
大沼貴之、深溝 慶 ：糖質とタンパク質の相互作用解析、『機能性糖質素材の開発と食品への応用Ⅱ』監修：井上國世、シーエムシー出版 (2013)、pp. 232-240
新家粧子、深溝 慶 ：ディスコイジン・ドメインの多様な結合特異性、キチン・キトサン研究Chitin and Chitosan Research, 2013, 19(1), 28-33
Umemoto N, Ohnuma T, Fukamizo T. Enzymatic synthesis of chitin oligosaccharides with longer chains. In Marine Medical Glycomics, Nova Science Publishers, Inc. pp. 39-58 (2013)
Shinya S, Ohnuma T, Brzezinski R, Scheerle RK, Grassmann J, Letzel T, and Fukamizo T. Lysozyme Superfamily: Progress in Functional Analysis using ESI-MS and NMR Spectroscopy, in Lysozymes: Sources, Functions and Role in Disease, ed. by Maang XG, Cheung WF, Nova Science Publishers, Inc. pp. 127-153 (2013)
深溝 慶、新家粧子: キトサナーゼの加水分解機構と基質認識機構、食品酵素化学の最新技術と応用Ⅱ―展開するフードプロテオミクス―、編集：井上國世、シーエムシー出版、pp. 58-69 (2011)
Ohnuma T, Fukamizo T Expression and Purification of Bioactive Proteins/Peptides with Conventional Liquid Chromatography. in Protein and Peptide Analysis by LC-MS: Experimental Strategies (RSC Chromatography Monographs) (ed. by Letzel T), RSC, Publishing pp.26-37(2011)
Ohnuma T, Fukamizo T Chitin/chitosan oligosaccharides: effective substrates for functional analysis of chitinases/ chitosanases. in Chitin, Chitosan and their derivatives: Biological activitie and Industrial applications (ed. by Se-Kwon Kim), CRC Press, pp.295-324 (2010)
大沼貴之，翁長彰子，深溝 慶，平良東紀: 特異な糖質結合モジュールLysMドメインの構造と機能. 化学と生物，47，676-677(2009)
深溝 慶，大沼貴之 : 糖質加水分解酵素における反応解析法の進展. 化学と生物，47，404-411(2009)
北奥喜仁, 大沼貴之 ：等温滴定型カロリメトリーを用いた生体分子間相互作用解析、化学と生物, 53(12)834-842 (2015)
大沼貴之 ：椊物キチナーゼの構造と機能、その利用、応用糖質科学, 4(2)121 (2014)
深溝 慶 ：キチン質の酵素分解とオリゴ糖合成研究の新展開―緒言―キチン質多糖の分解とオリゴ糖合成、応用糖質科学, 4(2)103-105 (2014)
新家粧子、深溝 慶 ：CBM32ファミリーに属する糖質結合モジュールの構造と特異性、キチン・キトサン研究Chitin and Chitosan Research, 2014, 20(1), 38-43
大沼貴之、深溝 慶 ：糖質とタンパク質の相互作用解析、『機能性糖質素材の開発と食品への応用Ⅱ』監修：井上國世、シーエムシー出版 (2013)、pp. 232-240
新家粧子、深溝 慶 ：ディスコイジン・ドメインの多様な結合特異性、キチン・キトサン研究Chitin and Chitosan Research, 2013, 19(1), 28-33
Umemoto N, Ohnuma T, Fukamizo T. Enzymatic synthesis of chitin oligosaccharides with longer chains. In Marine Medical Glycomics, Nova Science Publishers, Inc. pp. 39-58 (2013)
Shinya S, Ohnuma T, Brzezinski R, Scheerle RK, Grassmann J, Letzel T, and Fukamizo T. Lysozyme Superfamily: Progress in Functional Analysis using ESI-MS and NMR Spectroscopy, in Lysozymes: Sources, Functions and Role in Disease, ed. by Maang XG, Cheung WF, Nova Science Publishers, Inc. pp. 127-153 (2013)
深溝 慶、新家粧子: キトサナーゼの加水分解機構と基質認識機構、食品酵素化学の最新技術と応用Ⅱ―展開するフードプロテオミクス―、編集：井上國世、シーエムシー出版、pp. 58-69 (2011)
Ohnuma T, Fukamizo T Expression and Purification of Bioactive Proteins/Peptides with Conventional Liquid Chromatography. in Protein and Peptide Analysis by LC-MS: Experimental Strategies (RSC Chromatography Monographs) (ed. by Letzel T), RSC, Publishing pp.26-37(2011)
Ohnuma T, Fukamizo T Chitin/chitosan oligosaccharides: effective substrates for functional analysis of chitinases/ chitosanases. in Chitin, Chitosan and their derivatives: Biological activitie and Industrial applications (ed. by Se-Kwon Kim), CRC Press, pp.295-324 (2010)
大沼貴之，翁長彰子，深溝 慶，平良東紀: 特異な糖質結合モジュールLysMドメインの構造と機能. 化学と生物，47，676-677(2009)
深溝 慶，大沼貴之 : 糖質加水分解酵素における反応解析法の進展. 化学と生物，47，404-411(2009)
原著論文：
2016年
Shinya S, Nishimura S, Kitaoku Y, Numata T, Kimoto H, Kusaoke H, Ohnuma T, Fukamizo T. Mechanism of chitosan recognition by CBM32 carbohydrate-binding modules from a Paenibacillus sp. IK-5 chitosanase/glucanase.The Biochemical journal (in press)
Ohnuma T, Dozen S, Honda Y, Kitaoka M, Fukamizo T. A glycosynthase derived from an inverting chitinase with an extended binding cleft.Journal of biochemistry (in press)
Somboonpatarakun C, Shinya S, Kawaguchi Y, Araki T, Fukamizo T, Klaynongsruang S. A goose-type lysozyme from ostrich (Struthio camelus) egg white: multiple roles of His101 in its enzymatic reaction.Biosci Biotechnol Biochem. 80, 264-72
Sirimontree P, Fukamizo T, Suginta W. Azide anions inhibit GH-18 endochitinase and GH-20 Exo β-N-acetylglucosaminidase from the marine bacterium Vibrio harveyi.J Biochem. 159, 191-200
Shinya S, Nishimura S, Kitaoku Y, Numata T, Kimoto H, Kusaoke H, Ohnuma T, Fukamizo T. Mechanism of chitosan recognition by CBM32 carbohydrate-binding modules from a Paenibacillus sp. IK-5 chitosanase/glucanase.The Biochemical journal (in press)
Ohnuma T, Dozen S, Honda Y, Kitaoka M, Fukamizo T. A glycosynthase derived from an inverting chitinase with an extended binding cleft.Journal of biochemistry (in press)
Somboonpatarakun C, Shinya S, Kawaguchi Y, Araki T, Fukamizo T, Klaynongsruang S. A goose-type lysozyme from ostrich (Struthio camelus) egg white: multiple roles of His101 in its enzymatic reaction.Biosci Biotechnol Biochem. 80, 264-72
Sirimontree P, Fukamizo T, Suginta W. Azide anions inhibit GH-18 endochitinase and GH-20 Exo β-N-acetylglucosaminidase from the marine bacterium Vibrio harveyi.J Biochem. 159, 191-200
2015年
Umemoto N, Ohnuma T, Osawa T, Numata T, Fukamizo T. Modulation of the transglycosylation activity of plant family GH18 chitinase by removing or introducing a tryptophan side chain. FEBS letters 589(18)2327-2333
Kitaoku Y, Umemoto N, Ohnuma T, Numata T, Taira T, Sakuda S, Fukamizo T. A class III chitinase without disulfide bonds from the fern, Pteris ryukyuensis: crystal structure and ligand-binding studies. Planta 242, 895-907
Inamine S, Onaga S, Ohnuma T, Fukamizo T, Taira T. Purification, cDNA cloning, and characterization of LysM-containing plant chitinase from horsetail (Equisetum arvense). Biosci Biotechnol Biochem. 2015, 30, 1-9.
Umemoto N, Kanda Y, Ohnuma T, Osawa T, Numata T, Sakuda S, Taira T, Fukamizo T. Crystal structures and inhibitor binding properties of plant class V chitinases: the cycad enzyme exhibits unique structural and functional features. Plant J. 2015, 82, 54-66.
Umemoto N, Ohnuma T, Osawa T, Numata T, Fukamizo T. Modulation of the transglycosylation activity of plant family GH18 chitinase by removing or introducing a tryptophan side chain. FEBS letters 589(18)2327-2333
Kitaoku Y, Umemoto N, Ohnuma T, Numata T, Taira T, Sakuda S, Fukamizo T. A class III chitinase without disulfide bonds from the fern, Pteris ryukyuensis: crystal structure and ligand-binding studies. Planta 242, 895-907
Inamine S, Onaga S, Ohnuma T, Fukamizo T, Taira T. Purification, cDNA cloning, and characterization of LysM-containing plant chitinase from horsetail (Equisetum arvense). Biosci Biotechnol Biochem. 2015, 30, 1-9.
Umemoto N, Kanda Y, Ohnuma T, Osawa T, Numata T, Sakuda S, Taira T, Fukamizo T. Crystal structures and inhibitor binding properties of plant class V chitinases: the cycad enzyme exhibits unique structural and functional features. Plant J. 2015, 82, 54-66.
2014年
Nishihira T, Miyano A, Ohnuma T*, Gotoh T, Takahashi S, Narihiro K, Yamashita K, Fukamizo T. A Simple Turbidimetric Assay Using Chitin Nanofibers as the Substrate for Determinination of Chitinase Activity. J Appl Glycosci, 61, 4, 113-116
Sirimontree P, Suginta W, Sritho N, Kanda Y, Shinya S, Ohnuma T, Fukamizo T. Mutation strategies for obtaining chitooligosaccharides with longer chains by transglycosylation reaction of family GH18 chitinase. Biosci Biotechnol Biochem. 2014, 78, 2014-2021.
Shinya S, Urasaki A, Ohnuma T, Taira T, Suzuki A, Ogata M, Usui T, Lampela O, Juffer AH, Fukamizo T. Interaction of di-N-acetylchitobiosyl moranoline with a family GH19 chitinase from moss, Bryum coronatum. Glycobiology. 2014, 24, 945-955.
Ohnuma T, Umemoto N, Nagata T, Shinya S, Numata T, Taira T, Fukamizo T. Crystal structure of a "loopless" GH19 chitinase in complex with chitin tetrasaccharide spanning the catalytic center. Biochim Biophys Acta. 2014, 1844, 793-802.
Mahata M, Shinya S, Masaki E, Yamamoto T, Ohnuma T, Brzezinski R, Mazumder TK, Yamashita K, Narihiro K, Fukamizo T. Production of chitooligosaccharides from Rhizopus oligosporus NRRL2710 cells by chitosanase digestion. Carbohydr Res. 2014, 383, 27-33.
Nishihira T, Miyano A, Ohnuma T*, Gotoh T, Takahashi S, Narihiro K, Yamashita K, Fukamizo T. A Simple Turbidimetric Assay Using Chitin Nanofibers as the Substrate for Determinination of Chitinase Activity. J Appl Glycosci, 61, 4, 113-116
Sirimontree P, Suginta W, Sritho N, Kanda Y, Shinya S, Ohnuma T, Fukamizo T. Mutation strategies for obtaining chitooligosaccharides with longer chains by transglycosylation reaction of family GH18 chitinase. Biosci Biotechnol Biochem. 2014, 78, 2014-2021.
Shinya S, Urasaki A, Ohnuma T, Taira T, Suzuki A, Ogata M, Usui T, Lampela O, Juffer AH, Fukamizo T. Interaction of di-N-acetylchitobiosyl moranoline with a family GH19 chitinase from moss, Bryum coronatum. Glycobiology. 2014, 24, 945-955.
Ohnuma T, Umemoto N, Nagata T, Shinya S, Numata T, Taira T, Fukamizo T. Crystal structure of a "loopless" GH19 chitinase in complex with chitin tetrasaccharide spanning the catalytic center. Biochim Biophys Acta. 2014, 1844, 793-802.
Mahata M, Shinya S, Masaki E, Yamamoto T, Ohnuma T, Brzezinski R, Mazumder TK, Yamashita K, Narihiro K, Fukamizo T. Production of chitooligosaccharides from Rhizopus oligosporus NRRL2710 cells by chitosanase digestion. Carbohydr Res. 2014, 383, 27-33.
2013年
Ohnuma T, Umemoto N, Taira T, Fukamizo T, Numata T. Crystallization and preliminary X-ray diffraction analysis of an active-site mutant of `loopless' family GH19 chitinase from Bryum coronatum in a complex with chitotetraose. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Dec;69(Pt 12):1360-2.
Lacombe-Harvey MÈ, Fortin M, Ohnuma T, Fukamizo T, Letzel T, Brzezinski R. A highly conserved arginine residue of the chitosanase from Streptomyces sp. N174 is involved both in catalysis and substrate binding. BMC Biochem. 2013, 14:23.
Shinya S, Ohnuma T, Yamashiro R, Kimoto H, Kusaoke H, Anbazhagan P, Juffer AH, Fukamizo T. The first identification of carbohydrate binding modules specific to chitosan. J Biol Chem. 2013, 288, 30042-53.
Ohnuma T, Umemoto N, Kondo K, Numata T, Fukamizo T. Complete subsite mapping of a "loopful" GH19 chitinase from rye seeds based on its crystal structure. FEBS Lett. 2013, 587, 2691-7.
Arimori T, Kawamoto N, Shinya S, Okazaki N, Nakazawa M, Miyatake K, Fukamizo T, Ueda M, Tamada T. Crystal structures of the catalytic domain of a novel glycohydrolase family 23 chitinase from Ralstonia sp. A-471 reveals a unique arrangement of the catalytic residues for inverting chitin hydrolysis. J Biol Chem. 2013, 288, 18696-706
Ogata M, Umemoto N, Ohnuma T, Numata T, Suzuki A, Usui T, Fukamizo T. A Novel Transition-state Analogue for Lysozyme, 4-O-β-Tri-N-acetylchitotriosyl Moranoline, Provided Evidence Supporting the Covalent Glycosyl-enzyme Intermediate. J Biol Chem. 2013, 288, 6072-6082.
Wakinaka T, Kiyohara M, Kurihara S, Hirata A, Chaiwangsri T, Ohnuma T, Fukamizo T, Katayama T, Ashida H, Yamamoto K. Bifidobacterial α-galactosidase with unique carbohydrate-binding module specifically acts on blood group B antigen. Glycobiology. 2013, 23, 232-240.
Umemoto N, Ohnuma T, Mizuhara M, Sato H, Skriver K, Fukamizo T. Introduction of a tryptophan side chain into subsite +1 enhances transglycosylation activity of a GH-18 chitinase from Arabidopsis thaliana, AtChiC. Glycobiology. 2013, 23, 81-90.
Ohnuma T, Umemoto N, Taira T, Fukamizo T, Numata T. Crystallization and preliminary X-ray diffraction analysis of an active-site mutant of `loopless' family GH19 chitinase from Bryum coronatum in a complex with chitotetraose. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Dec;69(Pt 12):1360-2.
Lacombe-Harvey MÈ, Fortin M, Ohnuma T, Fukamizo T, Letzel T, Brzezinski R. A highly conserved arginine residue of the chitosanase from Streptomyces sp. N174 is involved both in catalysis and substrate binding. BMC Biochem. 2013, 14:23.
Shinya S, Ohnuma T, Yamashiro R, Kimoto H, Kusaoke H, Anbazhagan P, Juffer AH, Fukamizo T. The first identification of carbohydrate binding modules specific to chitosan. J Biol Chem. 2013, 288, 30042-53.
Ohnuma T, Umemoto N, Kondo K, Numata T, Fukamizo T. Complete subsite mapping of a "loopful" GH19 chitinase from rye seeds based on its crystal structure. FEBS Lett. 2013, 587, 2691-7.
Arimori T, Kawamoto N, Shinya S, Okazaki N, Nakazawa M, Miyatake K, Fukamizo T, Ueda M, Tamada T. Crystal structures of the catalytic domain of a novel glycohydrolase family 23 chitinase from Ralstonia sp. A-471 reveals a unique arrangement of the catalytic residues for inverting chitin hydrolysis. J Biol Chem. 2013, 288, 18696-706
Ogata M, Umemoto N, Ohnuma T, Numata T, Suzuki A, Usui T, Fukamizo T. A Novel Transition-state Analogue for Lysozyme, 4-O-β-Tri-N-acetylchitotriosyl Moranoline, Provided Evidence Supporting the Covalent Glycosyl-enzyme Intermediate. J Biol Chem. 2013, 288, 6072-6082.
Wakinaka T, Kiyohara M, Kurihara S, Hirata A, Chaiwangsri T, Ohnuma T, Fukamizo T, Katayama T, Ashida H, Yamamoto K. Bifidobacterial α-galactosidase with unique carbohydrate-binding module specifically acts on blood group B antigen. Glycobiology. 2013, 23, 232-240.
Umemoto N, Ohnuma T, Mizuhara M, Sato H, Skriver K, Fukamizo T. Introduction of a tryptophan side chain into subsite +1 enhances transglycosylation activity of a GH-18 chitinase from Arabidopsis thaliana, AtChiC. Glycobiology. 2013, 23, 81-90.
2012年
Ohnuma T, Numata T, Osawa T, Inanaga H, Okazaki Y, Shinya S, Kondo K, Fukuda T, Fukamizo T Crystal structure and chitin oligosaccharide-binding mode of a 'loopful' family GH19 chitinase from rye, Secale cereale, seeds. FEBS J, 19, 3639- 3651 (2012)
Ohnuma T, Taira T, Fukamizo T Antifungal activity of recombinant class V chitinases from Nicotiana tabacum and Arabidopsis thaliana. J Appl Glycosci, 59, 1, 47-50 (2012)
Umemoto N, Ohnuma T, Urpilainen H, Yamamoto T, Numata T, and Fukamizo T. Role of tryptophan residues in a class V chitinase from Nicotiana tabacum Biosci Biotechnol Biochem. 2012,76,778-784.
Ohnuma T, Fukuda T, Dozen S, Honda Y, Kitaoka M, Fukamizo T. A glycosynthase derived from an inverting GH19 chitinase from the moss Bryum coronatum. Biochem J. 2012,444,437-443.
Arakane Y, Taira T, Ohnuma T, Fukamizo T. Chitin-related enzymes in agro-biosciences. Curr Drug Targets. 2012,13,442-470.
Shinya S, Nagata T, Ohnuma T, Taira T, Nishimura S, Fukamizo T. Backbone chemical shifts assignments, secondary structure, and ligand binding of a family GH-19 chitinase from moss, Bryum coronatum. Biomol NMR Assign. 2012,6,157-161.
Ohnuma T, Numata T, Osawa T, Inanaga H, Okazaki Y, Shinya S, Kondo K, Fukuda T, Fukamizo T Crystal structure and chitin oligosaccharide-binding mode of a 'loopful' family GH19 chitinase from rye, Secale cereale, seeds. FEBS J, 19, 3639- 3651 (2012)
Ohnuma T, Taira T, Fukamizo T Antifungal activity of recombinant class V chitinases from Nicotiana tabacum and Arabidopsis thaliana. J Appl Glycosci, 59, 1, 47-50 (2012)
Umemoto N, Ohnuma T, Urpilainen H, Yamamoto T, Numata T, and Fukamizo T. Role of tryptophan residues in a class V chitinase from Nicotiana tabacum Biosci Biotechnol Biochem. 2012,76,778-784.
Ohnuma T, Fukuda T, Dozen S, Honda Y, Kitaoka M, Fukamizo T. A glycosynthase derived from an inverting GH19 chitinase from the moss Bryum coronatum. Biochem J. 2012,444,437-443.
Arakane Y, Taira T, Ohnuma T, Fukamizo T. Chitin-related enzymes in agro-biosciences. Curr Drug Targets. 2012,13,442-470.
Shinya S, Nagata T, Ohnuma T, Taira T, Nishimura S, Fukamizo T. Backbone chemical shifts assignments, secondary structure, and ligand binding of a family GH-19 chitinase from moss, Bryum coronatum. Biomol NMR Assign. 2012,6,157-161.
2011年
Fukamizo T, Sato H, Mizuhara M, Ohnuma T, Gotoh T, Hiwatashi K, Takahashi S. Chitinase from Autographa californica multiple nucleopolyhedrovirus: rapid purification from Sf-9 medium and mode of action. Biosci Biotechnol Biochem. 2011;75(9):1763-9.
Shinya S, Ohnuma T, Kawamura S, Torikata T, Nishimura S, Katoh E, Fukamizo T. Interaction of a goose-type lysozyme with chitin oligosaccharides as determined by NMR spectroscopy. J Biochem. 2011 Nov;150(5):569-77.
Ohnuma T, Sørlie M, Fukuda T, Kawamoto N, Taira T, Fukamizo T. Chitin oligosaccharide binding to a family GH19 chitinase from the moss Bryum coronatum. FEBS J. 2011 Nov;278(21):3991-4001.
Letzel T, Sahmel-Schneider E, Skriver K, Ohnuma T, Fukamizo T. Chitinase-catalyzed hydrolysis of 4-nitrophenyl penta-N-acetyl-β-chitopentaoside as determined by real-time ESIMS: The 4-nitrophenyl moiety of the substrate interacts with the enzyme binding site. Carbohydr Res. 2011 May 1;346(6):863-6.
Ohnuma T, Numata T, Osawa T, Mizuhara M, Lampela O, Juffer AH, Skriver K, Fukamizo T. A class V chitinase from Arabidopsis thaliana: gene responses, enzymatic properties, and crystallographic analysis. Planta. 2011 July;234(1):123-37.
Taira T, Mahoe Y, Kawamoto N, Onaga S, Iwasaki H, Ohnuma T, Fukamizo T. Cloning and characterization of a small family 19 chitinase from moss (Bryum coronatum). Glycobiology. 2011 May;21(5):644-54.
Ohnuma T, Numata T, Osawa T, Mizuhara M, Vårum KM, Fukamizo T. Crystal structure and mode of action of a class V chitinase from Nicotiana tabacum. Plant Mol Biol. 2011 Feb;75(3):291-304.
Fukamizo T, Sato H, Mizuhara M, Ohnuma T, Gotoh T, Hiwatashi K, Takahashi S. Chitinase from Autographa californica multiple nucleopolyhedrovirus: rapid purification from Sf-9 medium and mode of action. Biosci Biotechnol Biochem. 2011;75(9):1763-9.
Shinya S, Ohnuma T, Kawamura S, Torikata T, Nishimura S, Katoh E, Fukamizo T. Interaction of a goose-type lysozyme with chitin oligosaccharides as determined by NMR spectroscopy. J Biochem. 2011 Nov;150(5):569-77.
Ohnuma T, Sørlie M, Fukuda T, Kawamoto N, Taira T, Fukamizo T. Chitin oligosaccharide binding to a family GH19 chitinase from the moss Bryum coronatum. FEBS J. 2011 Nov;278(21):3991-4001.
Letzel T, Sahmel-Schneider E, Skriver K, Ohnuma T, Fukamizo T. Chitinase-catalyzed hydrolysis of 4-nitrophenyl penta-N-acetyl-β-chitopentaoside as determined by real-time ESIMS: The 4-nitrophenyl moiety of the substrate interacts with the enzyme binding site. Carbohydr Res. 2011 May 1;346(6):863-6.
Ohnuma T, Numata T, Osawa T, Mizuhara M, Lampela O, Juffer AH, Skriver K, Fukamizo T. A class V chitinase from Arabidopsis thaliana: gene responses, enzymatic properties, and crystallographic analysis. Planta. 2011 July;234(1):123-37.
Taira T, Mahoe Y, Kawamoto N, Onaga S, Iwasaki H, Ohnuma T, Fukamizo T. Cloning and characterization of a small family 19 chitinase from moss (Bryum coronatum). Glycobiology. 2011 May;21(5):644-54.
Ohnuma T, Numata T, Osawa T, Mizuhara M, Vårum KM, Fukamizo T. Crystal structure and mode of action of a class V chitinase from Nicotiana tabacum. Plant Mol Biol. 2011 Feb;75(3):291-304.
2010年
Ohnuma T, Osawa T, Fukamizo T, Numata T. Crystallization and preliminary X-ray diffraction analysis of a class V chitinase from Nicotiana tabacum. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Dec 1;66(Pt 12):1599-601.
Suginta W, Chuenark D, Mizuhara M, Fukamizo T. Novel β-N-acetylglucosaminidases from Vibrio harveyi 650: cloning, expression, enzymatic properties, and subsite identification. BMC Biochem. 2010 Sep 29;11:40.
Preecharram S, Jearranaiprepame P, Daduang S, Temsiripong Y, Somdee T, Fukamizo T, Svasti J, Araki T, Thammasirirak S. Isolation and characterisation of crocosin, an antibacterial compound from crocodile (Crocodylus siamensis) plasma. Anim Sci J. 2010 Jun;81(3):393-401.
Taira T, Fujiwara M, Dennhart N, Hayashi H, Onaga S, Ohnuma T, Letzel T, Sakuda S, Fukamizo T. Transglycosylation reaction catalyzed by a class V chitinase from cycad, Cycas revoluta: a study involving site-directed mutagenesis, HPLC, and real-time ESI-MS. Biochim Biophys Acta. 2010 Apr;1804(4):668-75.
Thammasirirak S, Pukcothanung Y, Preecharram S, Daduang S, Patramanon R, Fukamizo T, Araki T. Antimicrobial peptides derived from goose egg white lysozyme. Comp Biochem Physiol C Toxicol Pharmacol. 2010 Jan;151(1):84-91.
Ohnuma T, Osawa T, Fukamizo T, Numata T. Crystallization and preliminary X-ray diffraction analysis of a class V chitinase from Nicotiana tabacum. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Dec 1;66(Pt 12):1599-601.
Suginta W, Chuenark D, Mizuhara M, Fukamizo T. Novel β-N-acetylglucosaminidases from Vibrio harveyi 650: cloning, expression, enzymatic properties, and subsite identification. BMC Biochem. 2010 Sep 29;11:40.
Preecharram S, Jearranaiprepame P, Daduang S, Temsiripong Y, Somdee T, Fukamizo T, Svasti J, Araki T, Thammasirirak S. Isolation and characterisation of crocosin, an antibacterial compound from crocodile (Crocodylus siamensis) plasma. Anim Sci J. 2010 Jun;81(3):393-401.
Taira T, Fujiwara M, Dennhart N, Hayashi H, Onaga S, Ohnuma T, Letzel T, Sakuda S, Fukamizo T. Transglycosylation reaction catalyzed by a class V chitinase from cycad, Cycas revoluta: a study involving site-directed mutagenesis, HPLC, and real-time ESI-MS. Biochim Biophys Acta. 2010 Apr;1804(4):668-75.
Thammasirirak S, Pukcothanung Y, Preecharram S, Daduang S, Patramanon R, Fukamizo T, Araki T. Antimicrobial peptides derived from goose egg white lysozyme. Comp Biochem Physiol C Toxicol Pharmacol. 2010 Jan;151(1):84-91.
2009年
Taira T, Hayashi H, Tajiri Y, Onaga S, Uechi G, Iwasaki H, Ohnuma T, Fukamizo T. A plant class V chitinase from a cycad (Cycas revoluta): biochemical characterization, cDNA isolation, and posttranslational modification. Glycobiology. 2009 Dec;19(12):1452-61.
Dennhart N, Weigang LM, Fujiwara M, Fukamizo T, Skriver K, Letzel T. 26kDa endochitinase from barley seeds: real-time monitoring of the enzymatic reaction and substrate binding experiments using electrospray ionization mass spectrometry. J Biotechnol. 2009 Sep 25;143(4):274-83.
Takenaka Y, Nakano S, Tamoi M, Sakuda S, Fukamizo T. Chitinase gene expression in response to environmental stresses in Arabidopsis thaliana: chitinase inhibitor allosamidin enhances stress tolerance.. Biosci Biotechnol Biochem. 2009 May;73(5):1066-71.
Fukamizo T, Miyake R, Tamura A, Ohnuma T, Skriver K, Pursiainen NV, Juffer AH. A flexible loop controlling the enzymatic activity and specificity in a glycosyl hydrolase family 19 endochitinase from barley seeds (Hordeum vulgare L.). Biochim Biophys Acta. 2009 Aug;1794(8):1159-67.
Lacombe-Harvey ME, Fukamizo T, Gagnon J, Ghinet MG, Dennhart N, Letzel T, Brzezinski R. Accessory active site residues of Streptomyces sp. N174 chitosanase: variations on a common theme in the lysozyme superfamily. FEBS J. 2009 Feb;276(3):857-69.
Taira T, Hayashi H, Tajiri Y, Onaga S, Uechi G, Iwasaki H, Ohnuma T, Fukamizo T. A plant class V chitinase from a cycad (Cycas revoluta): biochemical characterization, cDNA isolation, and posttranslational modification. Glycobiology. 2009 Dec;19(12):1452-61.
Dennhart N, Weigang LM, Fujiwara M, Fukamizo T, Skriver K, Letzel T. 26kDa endochitinase from barley seeds: real-time monitoring of the enzymatic reaction and substrate binding experiments using electrospray ionization mass spectrometry. J Biotechnol. 2009 Sep 25;143(4):274-83.
Takenaka Y, Nakano S, Tamoi M, Sakuda S, Fukamizo T. Chitinase gene expression in response to environmental stresses in Arabidopsis thaliana: chitinase inhibitor allosamidin enhances stress tolerance.. Biosci Biotechnol Biochem. 2009 May;73(5):1066-71.
Fukamizo T, Miyake R, Tamura A, Ohnuma T, Skriver K, Pursiainen NV, Juffer AH. A flexible loop controlling the enzymatic activity and specificity in a glycosyl hydrolase family 19 endochitinase from barley seeds (Hordeum vulgare L.). Biochim Biophys Acta. 2009 Aug;1794(8):1159-67.
Lacombe-Harvey ME, Fukamizo T, Gagnon J, Ghinet MG, Dennhart N, Letzel T, Brzezinski R. Accessory active site residues of Streptomyces sp. N174 chitosanase: variations on a common theme in the lysozyme superfamily. FEBS J. 2009 Feb;276(3):857-69.
2008年
Fukamizo T, Hayashi K, Tamoi M, Fujimura Y, Kurotaki H, Kulminskaya A, Kitaoka M. Enzymatic hydrolysis of 1,3-1,4-beta-glucosyl oligosaccharides by 1,3-1,4-beta-glucanase from Synechocystis PCC6803: a comparison with assays using polymer and chromophoric oligosaccharide substrates. Arch Biochem Biophys. 2008 Oct 15;478(2):187-94.
Dennhart N, Fukamizo T, Brzezinski R, Lacombe-Harvey ME, Letzel T. Oligosaccharide hydrolysis by chitosanase enzymes monitored by real-time electrospray ionization-mass spectrometry. J Biotechnol. 2008 Apr 30;134(3-4):253-60.
Kawamura S, Chijiiwa Y, Minematsu T, Fukamizo T, Vårum KM, Torikata T. The role of Arg114 at subsites E and F in reactions catalyzed by hen egg-white lysozyme. Biosci Biotechnol Biochem. 2008 Mar;72(3):823-32
Mizuno R, Fukamizo T, Sugiyama S, Nishizawa Y, Kezuka Y, Nonaka T, Suzuki K, Watanabe T. Role of the loop structure of the catalytic domain in rice class I chitinase. J Biochem. 2008 Apr;143(4):487-95.
Fukamizo T, Hayashi K, Tamoi M, Fujimura Y, Kurotaki H, Kulminskaya A, Kitaoka M. Enzymatic hydrolysis of 1,3-1,4-beta-glucosyl oligosaccharides by 1,3-1,4-beta-glucanase from Synechocystis PCC6803: a comparison with assays using polymer and chromophoric oligosaccharide substrates. Arch Biochem Biophys. 2008 Oct 15;478(2):187-94.
Dennhart N, Fukamizo T, Brzezinski R, Lacombe-Harvey ME, Letzel T. Oligosaccharide hydrolysis by chitosanase enzymes monitored by real-time electrospray ionization-mass spectrometry. J Biotechnol. 2008 Apr 30;134(3-4):253-60.
Kawamura S, Chijiiwa Y, Minematsu T, Fukamizo T, Vårum KM, Torikata T. The role of Arg114 at subsites E and F in reactions catalyzed by hen egg-white lysozyme. Biosci Biotechnol Biochem. 2008 Mar;72(3):823-32
Mizuno R, Fukamizo T, Sugiyama S, Nishizawa Y, Kezuka Y, Nonaka T, Suzuki K, Watanabe T. Role of the loop structure of the catalytic domain in rice class I chitinase. J Biochem. 2008 Apr;143(4):487-95.
2007年
Tamoi M, Kurotaki H, Fukamizo T. Beta-1,4-glucanase-like protein from the cyanobacterium Synechocystis PCC6803 is a beta-1,3-1,4-glucanase and functions in salt stress tolerance. Biochem J. 2007 Jul 1;405(1):139-46.
Tamoi M, Kurotaki H, Fukamizo T. Beta-1,4-glucanase-like protein from the cyanobacterium Synechocystis PCC6803 is a beta-1,3-1,4-glucanase and functions in salt stress tolerance. Biochem J. 2007 Jul 1;405(1):139-46.
2006年
Sasaki C, Vårum KM, Itoh Y, Tamoi M, Fukamizo T. Rice chitinases: sugar recognition specificities of the individual subsites. Glycobiology. 2006 Dec;16(12):1242-50.
Fukamizo T, Fleury A, Côté N, Mitsutomi M, Brzezinski R. Exo-beta-D-glucosaminidase from Amycolatopsis orientalis: catalytic residues, sugar recognition specificity, kinetics, and synergism. Glycobiology. 2006 Nov;16(11):1064-72.
Takeuchi T, Morita K, Saito T, Kugimiya W, Fukamizo T. Chitosan-soyprotein interaction as determined by thermal unfolding experiments. Biosci Biotechnol Biochem. 2006 Jul;70(7):1786-9.
Côté N, Fleury A, Dumont-Blanchette E, Fukamizo T, Mitsutomi M, Brzezinski R. Two exo-beta-D-glucosaminidases/exochitosanases from actinomycetes define a new subfamily within family 2 of glycoside hydrolases. Biochem J. 2006 Mar 15;394(Pt 3):675-86.
Sasaki C, Vårum KM, Itoh Y, Tamoi M, Fukamizo T. Rice chitinases: sugar recognition specificities of the individual subsites. Glycobiology. 2006 Dec;16(12):1242-50.
Fukamizo T, Fleury A, Côté N, Mitsutomi M, Brzezinski R. Exo-beta-D-glucosaminidase from Amycolatopsis orientalis: catalytic residues, sugar recognition specificity, kinetics, and synergism. Glycobiology. 2006 Nov;16(11):1064-72.
Takeuchi T, Morita K, Saito T, Kugimiya W, Fukamizo T. Chitosan-soyprotein interaction as determined by thermal unfolding experiments. Biosci Biotechnol Biochem. 2006 Jul;70(7):1786-9.
Côté N, Fleury A, Dumont-Blanchette E, Fukamizo T, Mitsutomi M, Brzezinski R. Two exo-beta-D-glucosaminidases/exochitosanases from actinomycetes define a new subfamily within family 2 of glycoside hydrolases. Biochem J. 2006 Mar 15;394(Pt 3):675-86.
